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KMID : 0613820070170060772
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Journal of Life Science
2007 Volume.17 No. 6 p.772 ~ p.777
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Identification and expression of leuD Gene in Rice (Oryza sativa L.)
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Lee Eun-Tag
Kang Sang-Gu
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Abstract
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A rice OsLeuD gene for small subunit of 3-isopropylmalate isomerase (IPMI) (EC 4.2.1.33) has been isolated. OsLeuD gene is located on 109.3 cM of chromosome 2. OsLeuD gene was expressed abundantly in metabolically active organs including leaves and developing seeds, indicating that OsLeuD gene expression is developmentally regulated. The cDNA of OsLeuD gene was coded for 257 amino acids which showed 58% and 48% homology to small subunits of IPMI in LueD genes of cyanobacteria and green sulfur bacteria, respectively. The molecular character of OsLeuD is closely related to those of photosynthetic bacteria rather than those of eukaryotes including fungi and yeast. This suggests that OsLeuD gene in chromosomal genome of plants may possibly be originated from chloroplast genome.
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KEYWORD
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3-isopropylmalate isomerase, leucine biosynthesis, leuD, Oryza sativa
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